| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 207 | DRAPER, Hailey | SR | Roanoke |
2:45.00 | |
| 2 | 308 | BENTLEY, Baylee | SO | Concord |
3:05.00 | |
| 3 | 144 | MAST, Halie | SO | Eastern Mennonite |
2:29.65 | ODAC Outdoor Track & Field Championships |
| 4 | 142 | GARDINER, Vanessa | JR | Eastern Mennonite |
2:30.36 | ODAC Outdoor Track & Field Championships |
| 5 | 69 | LODGE, Kayleigh | SO | Columbia (S.C.) |
2:38.21 | Life University Indoor/Outdoor Invite |
| 6 | 724 | TUELL, Ashley | FR | Carson-Newman |
2:23.03 | |
| 7 | 141 | ASEFA, Elili | FR | Eastern Mennonite |
2:42.00 | |
| 8 | 480 | LLOYD, Malory | FR | Southern Virginia |
2:52.00 |