| Lane | # | Athlete | Age | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 419 | TAN, LE XIAO | 14 | Team Cicada Trackers | 28.67 | |
| 2 | 257 | ISHISE, KINO | 14 | Chij Secondary (Toa Payoh) | NT | |
| 3 | 433 | TOH, HUI JIE NATALYN | 14 | Chij St. Nicholas Girls' | NT | |
| 4 | 495 | CHAN, YU HUI GWENDOLYN | 13 | National Junior College | 32.13 | |
| 5 | 494 | THIRUMALAI, NIVEDITA | 13 | National Junior College | 35.61 | |
| 6 | 490 | ONG, YING RONG NICO | 15 | National Junior College | 30.01 | |
| 7 | 250 | WONG, CLARICE | 14 | Chij Secondary (Toa Payoh) | NT | |
| 8 | 491 | BINTE MOHAMMAD SHAH, NUR IRDINA | 14 | National Junior College | 31.24 |