| * | Facility | 4:09.02 | Upper Iowa | 2019 | |
| # | Mustang Open | 4:09.02 | Upper Iowa | 2019 | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 |  Mount Mercy | A | Garner | Woods | Schneider | Reittinger | 4:28.00 | 
| 2 |  Waldorf | A | Wilkinson | Mishleau | Clarke-Ashford | Perry | 4:23.16 | 
| 3 |  Cornell College | A | Osborn | Williams | Riss | Nava | 4:12.00 | 
| 4 |  Hawkeye CC | A | Lenaerts | Portch | Leicht | Hummell | 4:10.00 | 
| 5 |  Dubuque | A | Smith | Beeman | Innamorato | Miller | DNS | 
| 6 |  Viterbo | A | Conniff | Ihde | Yeakel | Caswell | 4:17.22 | 
| 7 |  St. Ambrose | A | Hart | Pierce | Hass | Ryan | 4:23.25 | 
| 8 |  Coe | A | Burke | Jesuit | Steege | Kittinger | DNS |