| * | Facility | 4:09.02 | Upper Iowa | 2019 | |
| # | Mustang Open | 4:09.02 | Upper Iowa | 2019 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Mount Mercy |
A | Garner | Woods | Schneider | Reittinger | 4:28.00 |
| 2 | Waldorf |
A | Wilkinson | Mishleau | Clarke-Ashford | Perry | 4:23.16 |
| 3 | Cornell College |
A | Osborn | Williams | Riss | Nava | 4:12.00 |
| 4 | Hawkeye CC |
A | Lenaerts | Portch | Leicht | Hummell | 4:10.00 |
| 5 | Dubuque |
A | Smith | Beeman | Innamorato | Miller | DNS |
| 6 | Viterbo |
A | Conniff | Ihde | Yeakel | Caswell | 4:17.22 |
| 7 | St. Ambrose |
A | Hart | Pierce | Hass | Ryan | 4:23.25 |
| 8 | Coe |
A | Burke | Jesuit | Steege | Kittinger | DNS |