| * | Facility | 42.94 | Coe | 2019 | |
| # | Mustang Open | 42.49 | Coe | 2019 | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 |  Cornell College | A | Engelken | O'Hara | Watters | Cowan | DNS | 
| 2 |  Viterbo | A | Savage | Jenkins | Lindstrom | Anderson | 44.99 | 
| 3 |  Mount Mercy | A | Powell | Christophersen | Bell | Harmon | 44.50 | 
| 4 |  St. Ambrose | B | Zachary | Rush | Polk | Davis | DNS | 
| 5 |  Hawkeye CC | A | Elliott | Tapia | Kajtezovic | Seth | 44.00 | 
| 6 |  Dubuque | C | Hardison | Byrd | Spires | Gabrys | 44.51 | 
| 7 |  Dubuque | D | Anderson | Alshomrani | Sigwarth | Atwater | DNS | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 |  Dubuque | B | Santiago | Wierick | Gohde | Griffin | 43.95 | 
| 2 |  North Iowa Area CC | A | Jackson | Jackson | McNight | Lee-Green | 43.00 | 
| 3 |  Iowa Central CC | B | Brown | Hicks-Jumper | Scott | Wilson | 42.50 | 
| 4 |  Iowa Central CC | A | Jessup | Hayles | Eppie | Dyer | 40.39 | 
| 5 |  Dubuque | A | Lentz | Oldham | Williams | Atwater | 42.37 | 
| 6 |  St. Ambrose | A | Olenek | Reemtsma | Reynolds | Reemtsma | 42.98 | 
| 7 |  Waldorf | A | Lopes | Thomas | Minor | Barrera | 43.50 | 
| 8 |  Coe | A | Weber | Rigdon | Fleck | Cory | 44.00 |