| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 | Independence | A | Ressler | Tanay | Decker | Mestad | 1:17.00 | 
| 3 | Benton Community | A | Heying | Heying | Wisner | Suiter | 1:08.53 | 
| 5 | Clear Creek Amana | A | Else | Haughey | Hardy | Rosenburg | 1:10.00 | 
| 7 | Center Point-Urbana | A | Sandberg | Dreier | Harford | Reed | 1:19.20 |