| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Clear Creek Amana | A | Burken | Overman | Strank | Vazquez | 4:55.00 | 
| 3 | Center Point-Urbana | A | Alcorn | Fleming | Nissen | Grimm | 4:41.60 | 
| 4 | Vinton-Shellsburg | A | Fisher | Johnson | Blais | Torres | 4:36.00 | 
| 5 | Benton Community | A | Junge | Volesky | Bieschke | Van Ashen | 4:39.17 | 
| 6 | Williamsburg | A | Stohlmann | Brummel | Weisskopf | Hirko | 4:47.00 | 
| 7 | Independence | A | Symonds | Kennedy | Christian | Szophinski | 5:00.00 |