| IA | Iowa HS Record | 41.33 | McMahon, Ringena, Richards, Coleman | Iowa City, City High | 1996 | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 3 | Postville | A | Hernandez | Salgado | Loera-Upton | Uhlenhake | 55.00 | 
| 4 | Kee High, Lansing | A | Goetzinger | Weber | Wagner | Cota | 49.37 | 
| 5 | Turkey Valley | A | Schmitt | Reicks | Schmitt | Busta | 49.81 | 
| 6 | West Central Maynard | A | Mathews | Allwood | Child | Streittmatter | 1:05.00 | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Central Elkader | A | Kelly | Wilwert | Orr | Weber | 49.15 | 
| 3 | MFL MarMac | A | Radloff | Powell | Breuer | Moses | 46.80 | 
| 4 | S Winn-Calmar | A | Kuboushek | Bushman | Conway | Herold | 44.71 | 
| 5 | North Fayette Valley | A | Rodgers | Wurzer | Turner | White | 46.25 | 
| 6 | Clayton Ridge | A | Cook | Scheber | Wahls | Palmer | 47.65 |