| IA | Iowa HS Record | 41.33 | McMahon, Ringena, Richards, Coleman | Iowa City, City High | 1996 | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Riceville | B | Fair | Keeling | Pridgen | Knode | 1:07.00 | 
| 3 | Dunkerton | B | Wolff | Reed | Boner | Hudson | 58.00 | 
| 4 | Central Springs | B | Walker | Christiansen | Prazak | Oliver | 47.98 | 
| 5 | Nashua-Plainfield | B | Wood | Franzen | Munn | White | 54.00 | 
| 6 | Turkey Valley | B | Hulbert | Kruse | Kleppe | Nieman | 56.00 | 
| 7 | Central Springs | C | Bleakney | Marker | Jose | Oliver | NT | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 | Janesville | A | Bromwich | Marcus | Wolff | Dodd | 53.00 | 
| 2 | Clarksville | A | Myers | Kampman | Bloker | Schmidt | 49.15 | 
| 3 | Nashua-Plainfield | A | White | Hillegas | Fundermann | Winters | 47.78 | 
| 4 | Central Springs | A | Molstad | Holding | Jacobsen | Pinske | 45.40 | 
| 5 | Dunkerton | A | VanLengen | Behrens | Kennedy | Gillespie | 46.17 | 
| 6 | Turkey Valley | A | Schmitt | Bodensteiner | Schmitt | Herold | 51.00 | 
| 7 | Riceville | A | Klaes | Swenson | Beran | Marr | 1:02.23 |