| IA | Iowa HS Record | 3:22.69 | Newell, Hill, Davis, Mims | Iowa City, City High | 2001 | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Riceville | A | Klaes | Fair | Fair | Beran | 4:33.85 | 
| 3 | Nashua-Plainfield | A | Fundermann | Winters | Munn | Lee | 4:06.14 | 
| 4 | Dunkerton | A | VanLengen | Gillespie | Behrens | Heideman | 3:55.00 | 
| 5 | Central Springs | A | Christiansen | Jacobsen | Mathers | Lanphere | 4:03.00 | 
| 6 | Turkey Valley | A | Bodensteiner | Schmitt | Busta | Reicks | 4:10.00 | 
| 7 | Clarksville | A | Bloker | Schmidt | Kampman | Ison | NT |