| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Midland | A | Hacke | Rupp | Bartels | Gatts | 4:45.00 | 
| 3 | Dubuque Hempstead | A | Curry | Becker | Heim | Lahey | 4:30.00 | 
| 4 | Monticello | A | Recker | Etzel | Tuetken | McQuillan | 4:25.00 | 
| 5 | Cascade | A | Gibbs | Simon | Knapp | Noonan | 4:28.00 | 
| 6 | Northeast | A | Fowler | Trenkamp | Petersen | Messerich | 4:40.00 | 
| 7 | Marion | A | Rompot | Rice | Fortman | Bartels | DQ |