| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Cascade | A | Koppes | Green | Knapp | Menster | 2:04.04 |
| 3 | Springville | A | March | Nachazel | Nealman | Howard | 1:59.20 |
| 4 | Western Dubuque | A | Schindler | Recker | Bahl | Biermann | 1:56.00 |
| 5 | Marion | A | Johnson | Poston | McAllister | Steffen | 1:58.00 |
| 6 | Central City | A | Rollinger | Ellis | Reid | Owens | 2:01.00 |
| 7 | Center Point-Urbana | A | Hansen | Kreel | Kramer | Maloney | 2:13.61 |