| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Central City | A | Kramer | Klostermann | Pruismann | Silver | 49.00 | 
| 3 | Cascade | A | Otting | Rickels | Alvarado | Finzel | 48.00 | 
| 4 | Easton Valley | A | Fuegen | Felkey | Fuegen | Murphy | DNS | 
| 5 | Center Point-Urbana | A | Griswald | Peterson | Wiley | Kramer | DQ | 
| 6 | Marion | A | Lovell | Hatangimana | Glade | Gardner | 48.20 | 
| 7 | Springville | A | Hoogland | Koppes | Menster | Brady | 52.00 |