| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Center Point-Urbana | A | Hunt | Kreel | Kramer | Ziltergruen | 2:03.63 | 
| 3 | Marion | A | Paulsen | Rice | Meth | McAllister | 2:00.00 | 
| 4 | Cascade | A | Gibbs | Koppes | Menster | Simon | 1:54.59 | 
| 5 | Western Dubuque | A | Schindler | Recker | Bahl | Biermann | 1:55.00 | 
| 6 | Springville | A | March | Brecht | Stamp | Blakely | 2:02.90 | 
| 7 | Central City | A | Noonan | Hennick | Ellis | Fritcher | 2:03.66 |