| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 | Central City | A | Greif | Olivas | Bechthold | Little | 5:00.00 | 
| 2 | East Buchanan | A | Fults | Bowers | Thurn | Thompson | 4:22.00 | 
| 3 | Springville | A | Hoogland | Koppes | DeMean | Robertson | 4:05.00 | 
| 4 | Easton Valley | A | Gruver | Franzen | Christof | Gruver | 3:55.00 | 
| 5 | Cascade | A | Rickels | Otting | Miller | Knepper | 4:00.00 | 
| 6 | Marion | A | Hatangimana | Gardner | Diers | Schmidt | 4:11.00 | 
| 7 | Center Point-Urbana | A | Gent | Hanson | Forman | Witte | 4:30.00 |