| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Center Point-Urbana | A | Anderson | Brinkman | Paine | Kirk | 5:45.00 | 
| 3 | Springville | A | Zaruba | Nachazel | Guerrero | Stejskal | 4:50.00 | 
| 4 | Marion | A | Johnson | Beltz | Steffen | Prier | 4:30.00 | 
| 5 | Central City | A | Rollinger | Fritcher | Reid | Owens | 4:38.70 | 
| 6 | Western Dubuque | A | Walters | Ramler | Willenborg | Mausser | 5:19.00 |