| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Jimtown | A | Ruffin | Martin | Bennett | Wiegand | 1:51.53 | Trine HSR Qualifier #1 |
| 4 | Elkhart | A | Williams | Horton | Barnes | Dale | 1:51.29 | Bulldog Invite - 4A-6A Teams - Morning Session |
| 5 | South Bend Adams | A | Kimbrough | Marsh | Williams | Perry | 1:45.62 | 2025 Indian Open |
| 6 | Warsaw Community | A | Stevens | Williams | Andrew | Burner | 1:46.69 | Taylor University HSR Qaulifie |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Westview | A | Litton | Miller | Weinberg | Wasson | 1:58.78 | New Haven Invite |
| 3 | Wabash | A | Wright | Alvarez | Jones | Bishir | 1:58.08 | IWU HSR Qualifier #3 |
| 4 | Lakeland | A | Bowman | Satterfield | Rettig | Hughes | 1:57.82 | 2024 Carmon Cripe Relays @ Northridge |
| 5 | LaPorte | A | Zapala | Anglin | Cushway | Campbell | NT | Trine HSR Qualifier #1 |
| 6 | Angola | A | Solis | Blue | Smith | Hakey | 1:57.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Prairie Heights | A | Corwin | Medina | Tuttle | Spessa | 2:20.00 | |
| 4 | Elkhart Christian | A | Jones | Jones | Vida | Grubert | NT | Trine HSR Qualifier #1 |
| 5 | Mississinewa | A | Rangel | Moore | Blake | Simmons | 2:01.49 | |
| 6 | Fairfield | A | Rink | Davis | McCauley | Reyes | 2:02.32 | Trine HSR Qualifier #2 |