| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Leo | A | Loomis | Zink | Treesh | Smith | 3:56.00 | |
| 4 | Ft. Wayne Blackhawk Christian | A | Grimes | Merchant | Kenner | Weigand | 3:50.00 | |
| 5 | Jimtown | A | Coughenour | Cole | Hamby | Inbody | 3:46.07 | John Glenn HS Dennis Rippy Invitational |
| 6 | Huntington North | A | Husband | Spahr | Shuler | Kowalski | 3:48.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Prairie Heights | A | Kurtz | Allred | Mason | Melson | 3:40.57 | Lakeland/Prairie Heights/Hamilton HS |
| 3 | Elkhart Christian | A | Neidhamer | Gentz | Curtis | Barnard | 3:40.00 | |
| 4 | Ft. Wayne Canterbury | A | Rohwer | O'Connor | Cvetkoski | Erb | 3:39.51 | Guerin Catholic Invitational |
| 5 | Angola | A | Hoover | Copeland | Rago | Stultz | 3:27.48 | 2025 NECC Championship Meet |
| 6 | Fairfield | A | Miller | Maust | Kio | Moreno | 3:39.02 | West Noble vs. Fairfield 4/29/25 |