| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | 
|---|---|---|---|---|---|---|---|---|
| 3 | Hauser | A | Estes | Kilps | Miller | Holzer | NT | |
| 4 | Elkhart Christian | A | Neidhamer | Ingle | Curtis | Liechty | NT | |
| 5 | Prairie Heights | A | Allred | Schlegel | Crites | Nott | DQ | Trine HSR Qualifier #3 | 
| 5 | Prairie Heights | A | Allred | Schlegel | Crites | Nott | DQ | Trine HSR Qualifier #3 | 
| 6 | Ft. Wayne Blackhawk Christian | A | Blosser | Fritcha | Goree | Weigand | 1:45.00 | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | 
|---|---|---|---|---|---|---|---|---|
| 3 | Fairfield | A | Moreno | Miller | Kaufman | Maust | 1:40.00 | |
| 4 | Angola | A | Blum | Koch | Phillips | Thomas | 1:36.96 | 2024 Goshen Relays - Boys - Class B | 
| 5 | Leo | A | Sauder | Crawford | Bingham | Miller | 1:35.64 | Heritage Relays | 
| 6 | Huntington North | A | Mitchell | Bordeaux | McQueen | Brooks | 1:36.00 |