| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Elkhart Christian | A | Alvarez | Glick | Whidden | Barnard | 9:11.18 | |
| 2 | Ft. Wayne Canterbury | A | Melo-Rolland | Kline | Collins | Hauze | 10:40.00 | |
| 3 | Jimtown | A | Steven | Marnell | Miller | Cuevas-Guapo | 9:05.97 | INSC Meet |
| 4 | Angola | A | Stultz | Hoover | Fletcher | Copeland | 8:02.75 | IHSAA Regional 2 (Boys) |
| 5 | Huntington North | A | Lippe | Cohen | Fishley | Eidson | 9:19.00 | |
| 6 | Leo | A | Foor | Bechtold | Powell | Trowbridge | 9:40.00 | |
| 7 | Fairfield | A | Cobern | Bontrager | Estep | Moreland | 8:29.93 | West Noble vs. Fairfield 4/29/25 |