| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Trine |
A | Birely | Forker | Possehn | Todd | 4:12.00 | |
| 2 | Calvin |
B | Wood | Hensel | Kolka | Ludwig | 4:10.00 | |
| 3 | Hope |
B | Kotsonis | Weseloh | Nemens | Sherman | 4:10.00 | |
| 4 | Calvin |
A | Davey | Bailey | Bossenbroek | Lambers | 4:03.50 | MIAA (DIII) Outdoor Track & Fi |
| 5 | Siena Heights |
A | Fisher | Fessler | McCulloch | Johnson | 3:52.41 | 2025 Hillsdale College Last Chance |
| 6 | Hope |
A | Cozzens | Klimp | Monville | Schermerhorn | 3:55.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Calvin |
D | DeBoer | DeJong | Tatko | Lingenfelter | 4:20.00 | |
| 2 | Ohio Northern |
A | Trusso | Denman | Lane | Vargo | 4:18.00 | |
| 3 | Albion |
A | Bennett | Kozal | Wells | Dwyer | 4:15.77 | Steemer Showcase |
| 4 | Trine |
B | Flores Ness | Peter | Wahl | Jackson | 4:15.00 | |
| 5 | Trine |
C | Valle | Young | Overmire | Puckett | 4:13.00 | |
| 6 | Calvin |
C | Schuurmann | Wierenga | Davids | Tubergen | 4:15.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Manchester |
B | Francis | Stetzel | Rominger | Paul | 4:40.00 | |
| 2 | Manchester |
A | Blakely | Danforth | Truelove | Jozsa | 4:40.00 | |
| 3 | Manchester |
C | Egolf | Norris | Cashdollar | Miller | 4:40.00 | |
| 4 | Albion |
B | Fenstermaker | Mata | Ames | Rogers | 4:30.13 | |
| 5 | Adrian |
A | Pentoney | Goldmeyer | Crowley | Townsend | 4:20.16 | MIAA (DIII) Outdoor Track & Fi |
| 6 | Hope |
C | Stuk | Sackrider | Jackson | Diekman | 4:24.00 |