| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mishawaka | B | Long | Buraczewski | Brugh | Rogers | 14:45.35 | Trine HSR Qualifier #1 |
| 2 | LaPorte | A | Spain | Conner | Bumgardner | Gillisse | 12:44.28 | 2022 NWI Championships |
| 3 | Angola | A | Budak | Chaisson | Thomas | Kazi | 14:00.00 | |
| 4 | Elkhart | A | Anderson | Johnson | Cruz-Portillo | Dibley | 14:15.00 | |
| 5 | Chesterton | B | Zhang | Haynes | Peterson | Wheele | 14:30.00 | |
| 6 | Elkhart | B | Eash | Hubbard | Becraft | Grasty | 15:00.00 | |
| 7 | Fremont | A | Gochenour | Baker | Gochenour | Shrewsburg | 14:38.22 | Hoosier State Relays Finals (Small Schools) |
| 8 | Mishawaka | A | Buraczewski | Fox | Bultinck | Flick | 14:45.35 | Trine HSR Qualifier #1 |
| 9 | Chesterton | A | Van Kley | Beumer | Wilgocki | Kroft | 14:00.00 | |
| 10 | Concord | A | Roberts | DeFreese | Potter | Latif | 15:05.50 |