| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Concord | A | Jones-Perry | Emerson | Cooper | DeFreese | 1:55.50 | |
| 2 | Chesterton | B | Morris | Geiser | Haggerty | Bamber | 1:52.00 | |
| 3 | South Bend Adams | A | Thomas | Soens | Douglas | Cruz | 1:46.00 | |
| 4 | Chesterton | A | Hurst | Kontos | Hardsock | Bradley | 1:49.00 | |
| 5 | Mishawaka | A | Deeds | Parks | Shelton | Naragon | 1:53.00 | |
| 6 | Mishawaka | B | Strohkirch | Leah | Naragon | Suggs | 1:55.58 | Trine HSR Qualifier #1 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Plymouth | A | Gurtner | Jones | Ameling | Joseph | 1:58.90 | |
| 2 | Fairfield | A | Stofleth | Dillon | Miller | Brown | 1:57.00 | |
| 3 | South Bend St. Joseph | A | Prokop | Goben | Wittig | Feldkamp | 1:56.77 | 2022 NWI Championships |
| 4 | Angola | A | Pelliccia | Gillen | Villa Romero | Davenport | 1:57.00 | |
| 5 | Northridge | A | Baugh | Mack | Soipan | Yeager | 1:57.62 | Trine HSR Qualifier #1 |
| 6 | LaPorte | A | Wilson | Conlin | Hertges | Anglin | 2:00.69 | 2022 NWI Championships |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Concord | B | Odhiambo | Herington | Wright | Odhiambo | 2:03.50 | |
| 2 | Elkhart | A | Williams | Brown | Wesselhoft | VanPelt | 2:02.72 | Trine HSR Qualifier #3 |
| 3 | Angola | B | Fletcher | DeLancey | Long | Sutton | 2:02.00 | |
| 4 | Fremont | A | Baker | Parr | Zuccolotto | Wills | 2:02.15 | Trine HSR Qualifier #3 |
| 5 | Elkhart | B | Addington | Higgins | Newson | Higgins | 2:02.72 | Trine HSR Qualifier #3 |
| 6 | Fairfield | B | Davis | Metz | Rink | Draper | 2:10.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lakeland | B | Gyovai | Granados | Alleshouse | Sanchez | 2:20.00 | |
| 3 | Plymouth | B | Contreras | Hindman | Garza | Ames | 2:10.00 | |
| 4 | Lakeland | A | Brown | Wallace | Ryan | Schiffli | 2:11.90 | Trine HSR Qualifier #3 |