| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Chesterton | A | Van Kley | Haynes | Wilgocki | McGrogan | NT | |
| 2 | Mishawaka | B | Bultinck | Brugh | Buraczewski | Long | 10:52.09 | Trine HSR Qualifier #1 |
| 3 | Elkhart | A | Anderson | Johnson | Cruz-Portillo | Dibley | 10:40.00 | |
| 4 | Chesterton | B | Kroft | Zhang | Peterson | Wheele | 10:45.00 | |
| 5 | Fairfield | A | Bechtel | Kuhn | Lambright | McCauley | 12:30.00 | |
| 6 | Mishawaka | A | Flick | Buraczewski | Rogers | Fox | 10:45.00 | |
| 7 | LaPorte | A | Hertges | Szumanski | Carnes | Poston | 10:28.02 | Trine HSR Qualifier #1 |
| 8 | Northridge | A | Baylis | Long | Long | Hile | 10:58.50 | |
| 9 | Concord | A | Davis | Plank | Salvador | Meribels | 11:00.00 | |
| 10 | Plymouth | A | Laird | Kara | Hissong | Wray | 11:37.03 | Trine HSR Qualifier #1 |
| 11 | South Bend St. Joseph | A | Collins | Hopkins | Ritschard | Frick | 10:45.00 | |
| 12 | Elkhart | B | Grasty | Vazquez | Perez | Eash | 12:30.00 |