| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Lynn |
B | 4:30.64 | STU Bobcat Season Opener |
| 4 | Ave Maria |
B | 4:35.00 | |
| 5 | St. Thomas Aquinas |
B | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Florida National | A | NT | |
| 2 | St. Thomas Aquinas |
A | 4:22.77 | |
| 3 | St. Thomas (Fla.) |
A | DNS | Sun Conference Outdoor Championship |
| 4 | Ave Maria |
A | 4:20.69 | SSAC/Sun Indoor Conference T&F Championships 2023 |
| 5 | Lynn |
A | 4:30.64 | STU Bobcat Season Opener |