| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | |
|---|---|---|---|---|---|---|---|---|
| 1 | Southeastern U. |
A | Williamson III | Taylor | Alamia | Worrels | 40.18 | South Florida Invitational |
| 2 | St. Thomas (Fla.) |
A | Weismore | Bowlin | Tanner | Shakespeare | 40.94 | 40.94 UCF Knights Invite |
| 3 | Keiser |
A | Desir | Christopher | Pritchett | Biget | 42.04 | UCF Knights Invite |
| 4 | Florida National | A | Dawson | Carrasco Rodriguez | Moonin | Woodside | 42.79 | STU Open 3 |
| 5 | Ave Maria |
A | Jenkins | Brooks | Van Horn | Kupcho | 43.46 | Keiser UniversityTwilight Invitational |
| 6 | Warner |
A | Vasquez | Brown | Gossett | Dawes | NT | |
| 7 | Webber |
A | Afolayan | Williams | Williams | Oquendo | NT |