| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Western |
B | Williams | Ruiz | Ruskin Jr | Charles | DNS | |
| 2 | West Broward |
A | Williamson | Etienne | Gay | Briggs | DNS | |
| 3 | Hollywood Hills |
A | ISAACS | DUMAS | POUGH | Golston | NT | |
| 4 | Chaminade-Madonna |
A | Cooper | Waters | Smith | Thomas | 41.95 | 41.95 BCAA South Central Qualifier |
| 5 | Chaminade-Madonna |
B | Gay III | Harris | Adras | Smith | 43.60 | |
| 6 | Hollywood Hills |
B | BAILEY | WHITE | William | Hadley | NT | |
| 7 | Western |
A | McBride | Suarez-Davis | Elliott | Exil | NT | |
| 8 | Western |
C | Jobes | Jean Marie | Petit | Gaddy | NT |