| ! | Records-All Classifications | 46.51 | George Washington HS | 2007 | State Track Meet | |
| ! | Records-All Classifications | 46.51 | Denver East HS | 2018 | State Track Meet |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Cedaredge High School | C | DQ | Verified | ||||
| 3 | Plateau Valley High School | A | Gutierrez-Gleason | Moore | Moore | Schroeder | 56.82 | Unverified: |
| 4 | Cedaredge High School | B | 55.70 | Unverified: | ||||
| 5 | Montrose High School | B | Leiba | McCracken | Walchle | Stephens | 54.00 | Unverified: |
| 6 | Olathe High School | A | Kimble | McCall | Almanza | Budde | 56.98 | Verified |
| 7 | Telluride High School | A | Rommel | Kramer | Saldin | Gianola | 1:00.27 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mancos High School | A | Mathews | Greenlee | Goodwin | Jabour | DQ | Verified |
| 2 | Montrose High School | A | Cato | Dillon | Hall | Labertew | NT | Unverified: |
| 3 | Coal Ridge High School | A | Herrera | Krueger | Wittenberg | Camunez | 52.45 | Verified |
| 4 | Delta High School | A | Wilson | Huff | Huggins | Fraser | 51.48 | Verified |
| 5 | Cedaredge High School | A | 51.70 | Verified | ||||
| 6 | Aspen High School | A | Zane | Khan-Farooqi | Thompson | Wesner | 52.91 | Verified |
| 7 | Caprock Academy High School | A | Herrera | Phillips | Chang | Reece | 54.07 | Verified |
| 8 | Grand Valley High School | A | Clegg | Sabata | Barragan | Speakman | 56.50 | Verified |