| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Crested Butte Middle School | A | 1:08.62 | Verified | ||||
| 4 | Cedaredge Middle School | A | Green | Brown | Mann | Robidoux | 55.15 | Verified |
| 5 | Columbine Middle School | A | 56.92 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ouray Middle School | B | Brown | Karsh | Kiparsky | Noel | 54.72 | Verified |
| 2 | Olathe Middle School | A | Young | Lynch | Sanchez | Fritze | 54.14 | Verified |
| 3 | Paonia Middle School | B | Kassoff | Holder | Etter | Morgan | 53.80 | Verified |
| 4 | Centennial (Montrose) Middle Sch | A | Pressler | Lovato | Bahn-Ricker | Mock | 51.54 | Verified |
| 5 | Paonia Middle School | A | Lee | McCrain | McCrain | Martina | 53.80 | Verified |
| 6 | Delta Middle School | A | Dickerson | Naranjo | McDaniel | Neurock | 54.13 | Verified |
| 7 | Olathe Middle School | B | Logan | McCall | Corn | Vincent | 54.14 | Verified |
| 8 | Ouray Middle School | A | Kinne | Young | Wold | Fiorito | 54.72 | Verified |