| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Chillicothe | A | King | Doss | Willison | Rinehart | 1:35:00.00 |
| 3 | Adena | A | Skeens | Colter-wolfe | Putnam | Dreitzler | 1:45.00 |
| 4 | Dawson-Bryant | A | Lucas | Simpson | Gipson | Ferguson | 1:34.27 |
| 5 | Southeastern | A | Dickson | Morgan | Hayburn | Smith | 1:42.00 |
| 6 | Zane Trace | A | Forcum | Parks | Pickerrell | Robinson | 1:50.00 |
| 7 | Waverly | A | McCain | Adkins | Riggs | Marquez | NT |