| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Adena | A | Smith | McWhorter | Skeens | Wright | 1:46.00 |
| 3 | Southeastern | A | George | Lenox | Evans | Smith | 1:38.00 |
| 4 | Huntington Local | A | Clay | McCloskey | Puckett | Haubeil | 1:37.50 |
| 5 | Paint Valley | A | Chambers | Hall | Perrone | Byrd | 1:38.00 |
| 6 | Leesburg-Fairfield | A | Teeters | Haines | Vidourek | Frost | 1:45.00 |
| 7 | Zane Trace | A | Dyke | Forcum | Fry | McDowell | 1:49.50 |