| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Southeastern | A | George | Coleman | Evans | Coonrod | 4:37.30 |
| 2 | Adena | A | Whitley | Kruger | Skeens | Wright | 4:09.00 |
| 3 | Leesburg-Fairfield | A | Teeters | Haines | Vidourek | Frost | 3:54.64 |
| 4 | Zane Trace | A | Jonhson | Hartsaugh | Vick | Clyne | 3:41.50 |
| 5 | Huntington Local | A | Hinshaw | McCloskey | Puckett | Haubeil | 3:45.00 |
| 6 | Paint Valley | A | Duhman | Pence | Blaney | Salomone | 4:00.00 |
| 7 | Crooksville | A | Osborne | Hunter | Hunter | Frash | 4:35.00 |