| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Zane Trace | A | Dyke | Forcum | Fry | McDowell | 48.63 |
| 3 | Southeastern | A | George | Coleman | Stewart | Evans | DQ |
| 4 | Paint Valley | A | Chambers | Hall | Perrone | Byrd | 46.00 |
| 5 | Huntington Local | A | Haubeil | Bellar | Marion | McIntosh | 47.00 |
| 6 | Adena | A | Smith | McWhorter | Stark | Skeens | 48.60 |