| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Urbana | A | Bradshaw | Wisma | Davis | Key | NT |
| 4 | Bellefontaine | A | Hutchins | Patel | Scott | Varner | 1:37.00 |
| 5 | Kenton Ridge | A | Brown | Hale | Patel | Stacy | 1:44.03 |
| 6 | Northwestern | A | Napier | Bishop | Fissel | Ernst | NT |
| 7 | Bellefontaine | B | NT |