| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Paint Valley | A | Smith | Vaughn | Dye | Mettler | 2:04.00 |
| 3 | Adena | A | Hurless | Harper | Burns | Martin | 2:01.70 |
| 4 | Unioto | A | Pelletiere | Heath | Seals | Storts-Berio | 1:55.00 |
| 5 | Southeastern | A | Wallace | Stotridge | Mitten | Jones | 2:01.60 |
| 6 | Zane Trace | A | Hill | Lane | Prochaska | Wade | 2:03.00 |
| 7 | Huntington Local | A | Ater | Griffin | Kifer | Oyer | 2:04.30 |