| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Adena | A | Smith | Throckmorton | McWhorter | Skeens | 1:40.20 |
| 3 | Unioto | A | Lohnes | McBee | Dollison | Beverly | 1:39.00 |
| 4 | Huntington Local | A | Clay | McCloskey | Richards | Puckett | 1:37.50 |
| 5 | Southeastern | A | George | Coleman | Coonrod | Stewart | 1:38.00 |
| 6 | Paint Valley | A | Chambers | Hall | Duhman | Byrd | 1:40.00 |
| 7 | Zane Trace | A | Dyke | Fry | Forcum | Langley | 1:42.52 |