| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Pickerington North | B | Cain | Timsina | Mcdougal | Culver | NT |
| 2 | Pickerington North | C | Hudak | Boateng | Kwarteng Duah | Temenu | NT |
| 3 | Reynoldsburg | A | Love | Towns | Gibson | Felts-Dingess | 53.59 |
| 4 | Bexley | A | Derrow | Brickerson | Flowers | Shyshkova | 51.84 |
| 5 | Pickerington North | A | Garcia | Crumpton | Samczak | Mekonnen | 52.49 |
| 6 | Groveport Madison | A | Bell | Thierry | Jones | Yancey | 1:58.00 |
| 7 | Pickerington North | D | Acharya | Forsyth | Bonsu | Edgerton | NT |