| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Groveport Madison Junior | A | Thomas | Hayes | Golden | Johnson | 53.54 |
| 4 | Pickerington Lakeview | A | Taylor | Taylor | Washington | Robinson | 46.87 |
| 5 | Canal Winchester | A | Harris | Felts | King | Kirk | 52.64 |
| 6 | Pickerington Lakeview | B | Bowden | Meyer | Johnson | Williams | NT |