| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 | Worthington Kilbourne | B | Terrell | Howard | Korn | McNeill | DQ | 
| 3 | St Francis De Sales-Columbus | A | Amonor | Crabtree | Karimy | Assibey | 1:40.00 | 
| 4 | Olentangy Liberty | A | Kocher | Lloyd | Monroe | Nelson | 1:31.96 | 
| 5 | Worthington Kilbourne | A | Craig | Darner | Konadu | Fuller | 1:34.75 | 
| 6 | St Francis De Sales-Columbus | B | Amalaman | Omameh | Sanon | Anderson | NT |