| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Gahanna East | A | Smith | McGinn | Thomas | Bentley | 58.40 |
| 2 | New Albany | A | Toler | Bounemany | Hall | Iaquinta | 57.62 |
| 3 | Gahanna South | A | Holmes | Eggleston | Davis | Wright | 55.00 |
| 4 | Gahanna West | A | Whitfield | Lewis | Whitfield | Brown | 53.00 |
| 5 | Pickerington Ridgeview | A | Kendrix | Plair | Smith | Miles | 54.00 |
| 6 | Genoa | A | Watkins | Gilbert | Brittman | King | 55.70 |
| 7 | Heritage (Westerville) | A | Sesay | Lytus | Smith | Mollett | 58.11 |