| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Iola Middle School | A | Boren | Drago | Hutton | Lampe | 1:03.30 |
| 3 | Anderson County Middle School | A | Smith | Steele | Wheat | Barnett | 49.97 |
| 4 | Santa Fe Trail Middle School | A | Williams | Hinck | Crotinger | Johnson | 48.20 |
| 5 | Wellsville Middle School | A | Otwell | Douglas | Strenth | Scott | 49.64 |
| 6 | Osawatomie Middle School | A | 53.56 |