| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Iola Middle School | A | NT | ||||
| 2 | Osawatomie Middle School | A | NT | ||||
| 3 | Wellsville Middle School | A | 2:22.70 | ||||
| 4 | Anderson County Middle School | A | Morrow | Brady | Miller | Katzer | 2:15.35 |
| 5 | Burlington Middle School | A | 2:22.00 | ||||
| 6 | Santa Fe Trail Middle School | A | Smith | Pickering | Johnson | Huffman | 5:32.83 |
| 7 | Prairie View Middle School | A | Case | Holler | Swearingen | Hightower | NT |