| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Anderson County High School | A | 5:24.75 | ||||
| 4 | Chanute High School | A | 4:38.00 | ||||
| 4 | Chanute High School | A | 4:38.00 | ||||
| 5 | Humboldt High School | A | Hunter | Jones | Lassman | Goforth | 4:55.50 |
| 6 | Iola High School | A | Abigail | Coltrane | Lohman | Williams | 5:08.14 |