| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | WHEATON Middle School | A | Bui | Cha | Peterson | Dutra | 1:11.07 |
| 3 | Thomas Jefferson Independent Sch | A | Green | Appuhamy | Mokhtar | Radius | 51.80 |
| 4 | St. Peters Catholic (Joplin) | A | Mirdamadi | Buccieri | Williams | Williams | 1:00.07 |