| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Joplin High School | A | Gilmore | Mack | Sotlar | Woods | 48.56 |
| 2 | Carthage High School | A | Contreras | Ralston | Carreto Martinez | Escobar | 47.06 |
| 3 | McDonald County High School | A | Bartley | PAXTOR | Feagin | LOPEZ VASQUEZ | NT |
| 4 | Neosho High School | A | Farley | Cote | Meyer | Suldan | 47.00 |
| 5 | Webb City High School | A | Dexter Pascua | McDaniel | Nichols | Burkhart | 47.41 |
| 6 | Diamond High School | A | Belcher | Roughton | King | Meza | 49.35 |