| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | |
|---|---|---|---|---|---|---|---|
| 1 | McDonald County High School | A | LOPEZ VASQUEZ | PAXTOR | Rubeck | Feagin | 1:34.99 |
| 2 | Carthage High School | A | Contreras | Ralston | Carreto Martinez | Escobar | 1:38.70 |
| 3 | Diamond High School | B | Belcher | Meza | King | Roughton | 1:39.93 |
| 4 | Neosho High School | A | Farley | Rosiere | Meyer | Day | 1:40.00 |
| 5 | East Newton High School | A | Larsen | Powell | Thornton | Silva | 1:43.87 |
| 6 | Joplin High School | A | Gilmore | Mack | Sotlar | Woods | 1:45.35 |
| 7 | Webb City High School | A | Dexter Pascua | McDaniel | Logan | Reid Jr | 1:47.22 |