| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Wyoming |
A | Drube | Harvey | Mendoza-Molina | Vincent | 4:00.00 | |
| 2 | A | Lopez | Davis | Martinez | Hawk | 3:54.24 | raw:3:53.801 CSU Pueblo Open | |
| 3 | Colorado |
A | Moorer | Glynn | Pollak | Renz | 3:33.54 | NCAA West Preliminary Round |
| 4 | Air Force |
A | Leverenz | Rioux | Aittama | Bodine | 3:47.93 | 2023 Mountain West Outdoor Track & Field Championships |
| 5 | Air Force |
B | Fraga | Iwu | Fereday | Loida | 3:55.00 | |
| 6 | Northern Colorado |
A | Hafner | Nowels | Perales | Kemp | 4:05.00 |