| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HARDESTY, Aani | SO | Colo. Sch. of Mines |
57.80 | raw:57.69 Colorado Invitational |
| 2 | GALVIN, Grace | SR | Colo. Sch. of Mines |
57.28 | raw:57.17 Chuck Haering Classic |
| 3 | SOWELL, Naleia | SR | MSU Denver |
54.41 | raw:54.30 2024 RMAC Outdoor Track & Field Championship |
| 4 | LENEWEAVER, Aubrey | SO | Colorado |
56.85 | Virginia Challenge |
| 5 | COMER, Allison | SR | Colo. Sch. of Mines |
57.66 | 9th Annual Indoor Gorilla Classic |
| 6 | EVANS, Maya | FR | Colo. Sch. of Mines |
57.99 |