| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Colorado Mines |
A | Beach | Kelly | Nelson | Moriarty | 3:51.16 | raw:3:50.721 Mines Kit Mayer Classic |
| 4 | CSU-Pueblo |
A | Case | Hernandez | Streff | Fairchild | 3:50.85 | raw:3:50.411 WTAMU Joe Meaker Classic and Multi |
| 5 | Western Colorado |
A | Firor | Portis | Burt | Sharon | 3:48.00 | |
| 6 | A | Benton | Glover | Tracy | Wenham | 3:49.08 | raw:3:48.641 Maverick Multi & Open | |
| 7 | Black Hills St. |
A | Dykstra | Bates | Lundquist | Tracy | 4:03.00 |