| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | SCOTT, Kamryn | SO | MSU Denver |
26.18 | raw:26.11 CSU-Pueblo Open/Early Bird |
| 4 | ROMERO, Allyssa | FR | MSU Denver |
25.92 | raw:25.85 CSU Pueblo Spank Blasing/Open |
| 5 | BOLTON, Keyanda | FR | 25.01 | raw:24.94 WTAMU Joe Meaker Classic and Multi | |
| 6 | FULLER, Breanne | SO | Black Hills St. |
25.15 | raw:25.08 2021 Yellow Jacket Spring Open |
| 7 | JOHNSON, Shynesti | SO | Western Colorado |
26.08 | raw:26.01 Colorado Invitational 2019 |
| 8 | POLLOCK, Natalie | FR | Colorado Mines |
26.69 | raw:26.62 CSU-Pueblo Open/Early Bird |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | JONES, Mikayla | FR | CSU-Pueblo |
28.72 | raw:28.58 Adams State Grizzlies Open |
| 4 | MILLER, Bella | FR | Western Colorado |
27.31 | raw:27.24 Maverick Sun Classic |
| 5 | KARCZEWSKI, Katie | FR | MSU Denver |
27.98 | raw:27.91 Mines Kit Mayer Classic |
| 6 | GUECK , Elizabeth | FR | Colo. Christian |
28.32 | raw:28.25 Maverick Sun Classic |