| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FRAGA, Amanda | FR | Air Force |
1:01.27 | raw:1:01.16 Jerry Quiller Classic 2021 |
| 2 | HENNIGAR, Peri | FR | Wyoming |
59.50 | |
| 3 | NELSON, Amena | FR | Colorado Mines |
58.51 | raw:58.40 Doug Max Invitational |
| 4 | HIGASHI, Randi | FR | Colorado Mines |
57.71 | raw:57.60 CSU Pueblo Spank Blasing/Open |
| 5 | MORIARTY, Layla | JR | Colorado Mines |
58.14 | raw:58.03 CSU Pueblo Spank Blasing/Open |
| 6 | RAMIREZ, Jenae | SO | Wyoming |
59.31 | raw:59.20 Doug Max Invitational |
| 7 | GONZALES-GIBBS, Kiona | FR | Wyoming |
59.52 | raw:59.41 Doug Max Invitational |
| 8 | SHOOPMAN, Sabrina | FR | Wyoming |
1:06.20 | raw:1:05.99 Air Force Invitational |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FOSTER, Camille | FR | Wyoming |
57.00 | |
| 2 | HURTA, Sage | SR | Colorado |
56.50 | |
| 3 | GOLDSWORTHY, Grace | FR | Colorado St. |
55.34 | raw:55.23 Doug Max Invitational |
| 4 | OZOUDE, Jessica | JR | Colorado St. |
53.90 | raw:53.79 Doug Max Invitational |
| 5 | GLYNN, Abbey | FR | Colorado |
54.01 | 2020 MPSF Indoor Track & Field Championships |
| 6 | DRAME, Jaida | JR | Colorado |
56.11 | raw:56.00 Doug Max Invitational |
| 7 | GREGORY, RubyAnn | JR | Northern Colorado |
56.87 | |
| 8 | CROSMER, Samantha | JR | Colorado |
57.09 | raw:56.98 Doug Max Invitational |